Tag
secretion

Part:BBa_K128004:Design

Designed by: Sara Mouradian   Group: iGEM08_MIT   (2008-07-18)


signal sequence from Lactobacillus bulgaricus; secretes protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cleavage site may be influenced by the protein that is attached to the signal peptide. To make sure that the protein does not get cleaved by mistake, four residues are added to the signal peptide sequence found by the Germond paper.

Signal peptides are almost always used in conjunction with another protein so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible



Source

It was isolated from the 5' end of the prtB gene in Lactobacillus bulgaricus, which codes for a lactose digesting protein that is bound to the surface (Germond JE et al.; Appl Environ Microbiol. 2003). This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

References